Microbiomes are of key importance at different scales in our society, ranging from individual-based health issues related to microbiomes inhabiting our body, to global greenhouse gas (i.e. CH4 and N2O) emissions. With UNLOCK, Wageningen University and Delft University of Technology have joined forces to implement and integrate experimental and data platforms, enabling breakthrough research and knowledge sharing on natural and synthetic microbial communities. UNLOCK is open to excellence-driven users from universities, knowledge institutes and industries, placing them in the unique position to conduct research at unmet speed and resolution. During the lecture, concepts on how to best explore, (re)design and deploy microbiomes will be presented, and examples given from various fields of application.